AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway

B a c k g r o u n d : A K o r e a n h e r b a l f o r m u l a t i o n , Ojayeonjonghwan, is used to treat late-onset hypogonadism (LOH) symptoms including erect i le dysfunct ion (ED). A previous research suggested that a modified Ojayeonjonghwan (KH-204) could be used as an alternative to treatment for ED. Pharmacological effects of KH-204 were examined in different conditions, including in vitro and vivo (an androgen-deprived rat model). Methods: We measured the survival rate of TM3 Leydig cells treated by KH-204 under the oxidative stress condition. The s.c. injection of leuprorelin was used to induce the androgen-deprivation. We removed and weighed the testes and epididymides, which would be used to histopathological examination, from the rats in experimental and control groups. Furthermore, we also measured serum testosterone levels, oxidative stress and apoptosis. Results: The results of treated by KH-204: (I) preserved TM3 cells from oxidative stress by improving the expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1); (II) lowered the expression of Transforming growth factor-beta (TGF-β) 1/ SMAD; (III) increased the average of serum testosterone in androgen-deprived male rats; (IV) kept the activation of spermatogenesis; (V) upgraded contents of 8-hydroxy20-deoxyguanosine (8-OHdG) and degraded contents of superoxide dismutase (SOD); and (VI) meaningfully reduced apoptosis. Conclusions: We studied the efficacy of KH-204 as an alternative therapy to improve testicular dysfunction in LOH. The efficacies of KH-204 are likely, at least in part, to degrade oxidative stress through the Nrf2/HO-1 pathway. These findings may offer credible evidences for the use of new complementary and alternative therapies to treat LOH.